The edited book consolidates information for profitable commercial cultivation of medicinal mushrooms. The book suggests many substrates for growers to use in the commercial cultivation of mushrooms. It also elucidates the conservation of wild endangered medicinal mushrooms. Mushrooms are fungal fruiting bodies seen by the naked eye and collected by hands. These are highly heterogeneous organisms characterized by high species diversity levels and widespread in all environments. Research conducted by a score of mycologists and biotechnologists has resulted in the continuous discovery of new species and the variability of environments where fungi can be harvested, including air, space, and the seabed. The fields of applications are unfolding a panorama of uses in varied fields, ranging from agriculture, bioremediation, forestry, food, cosmetics, medical, and pharmaceutical sectors. The book comprises three parts, first mentions their applications in Ayurvedic and traditional system of Chinese medicine for the cure of ailments. The truffles are delicious, while many others are recommended as a cure for deadly diseases like cancer, COVID-19, and HIV, as well as memory and longevity enhancer. Lentinus, Ganoderma, and Cordyceps are considered suitable as antioxidants and cures for inflammation. The second part deals with their occurrence in different habitats and seasons and their biology. Enzymes and mechanisms involved in biodegradation and anatomical details of rotting wood. The third part brings about the need for mushroom technology to improve the rural economy. This book is helpful for researchers and students in agriculture, agronomy, and research on mushrooms. ​