The presentation includes frequent cross-references to highlight the many connections between different techniques. The book provides a bird’ s-eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. It is a handy resource for students and researchers setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage means there is also a great deal of new material, even for experienced experimentalists.
The following techniques are covered in detail:
– Purification and determination of proteins
– Measuring enzymatic activity
– Microcalorimetry
– Immunoassays, affinity chromatography and other immunological methods
– Cross-linking, cleavage, and chemical modification of proteins
– Light microscopy, electron microscopy, and atomic force microscopy
– Chromatographic and electrophoretic techniques
– Protein sequence and composition analysis
– Mass spectrometry methods
– Measuring protein-protein interactions
– Biosensors
– NMR and EPR of biomolecules
– Electron microscopy and X-ray structure analysis
– Carbohydrate and lipid analysis
– Analysis of posttranslational modifications
– Isolation and determination of nucleic acids
– DNA hybridization techniques
– Polymerase chain reaction techniques
– Protein sequence and composition analysis
– DNA sequence and epigenetic modification analysis
– Analysis of protein-nucleic acid interactions
– Analysis of sequence data
– Proteomics, metabolomics, peptidomics and toponymic
– Chemical biology
